Spectrophotometer (UV-Vis) Agilent Cary 60

Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UV/Vis) refers to absorption spectroscopy in the ultraviolet-visible spectral region. This means it uses light in the visible and adjacent (near-UV and near-infrared (NIR)) ranges. The absorption in the visible range directly affects the perceived color of the chemicals involved. In this region of the electromagnetic spectrum, molecules undergo electronic transitions. UV/Vis spectroscopy is routinely used in the quantitative determination of solutions of transition metal ions highly conjugated organic compounds, and biological macromolecules.